recombinant β-type nrg1  (ProSpec)

Journal: Anatomy & Cell Biology

Article Title: Neuregulin 1/ErbB4 signaling attenuates neuronal cell damage under oxygen-glucose deprivation in primary hippocampal neurons

doi: 10.5115/acb.19.210

Figure Lengend Snippet: Neuregulin-1 (NRG1) attenuates apoptosis induced by oxygen-glucose deprivation (OGD) in primary hippocampal neurons. (A) After 2 hours, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining (red) was performed to determine the amount of apoptosis that occurred after OGD in primary hippocampal neurons treated with either vehicle or 5 nM NRG1. Hoechst staining (blue) was used as a counterstain. Scale bars=50 µm. (B) Numbers of TUNEL-positive cells (red) were quantified in 16 photographs from three independent experiments. The apoptotic neuronal cell ratio was expressed as a percent of TUNEL-positive cells to the total number of DAPI-positive cells (n=6, ** P <0.01, ## P <0.01).

Article Snippet: The NRG1 used in this study was a recombinant polypeptide containing the entire EGF domain of the β-type NRG1 from PROSPEC (East Brunswick, NJ, USA).

Techniques: TUNEL Assay, Staining

Journal: Anatomy & Cell Biology

Article Title: Neuregulin 1/ErbB4 signaling attenuates neuronal cell damage under oxygen-glucose deprivation in primary hippocampal neurons

doi: 10.5115/acb.19.210

Figure Lengend Snippet: Neuregulin-1 (NRG1) inhibits oxygen-glucose deprivation (OGD)-induced overexpression of ErbB4 in primary hippocampal neurons. (A) NRG1 prevents the elevation of ErbB4 receptor in OGD-induced cultured hippocampal neurons. Neurons were incubated with or without OGD for 2 hours in the presence or absence of pretreatment with NRG1 (5 nM) at 15 minutes prior to OGD. (B) Quantification of data in A. Densitometry values are shown as ratios relative to values of the control group (n=5, * P <0.05, # P <0.05). (C) Expression levels of ErbB4 after 2 hours of incubation under control, OGD alone, or OGD with pretreatment with 5 nM NRG1 in primary hippocampal neurons by immunofluorescence. Cells were double-labeled with DAPI (blue) and anti-ErbB4 (green) and then analyzed by fluorescence microscopy. Scale bars=50 µm. (D) The fluorescence density of ErbB4 was measured in each group (n=6, ** P <0.01, # P <0.05).

Article Snippet: The NRG1 used in this study was a recombinant polypeptide containing the entire EGF domain of the β-type NRG1 from PROSPEC (East Brunswick, NJ, USA).

Techniques: Over Expression, Cell Culture, Incubation, Control, Expressing, Immunofluorescence, Labeling, Fluorescence, Microscopy

Journal: Anatomy & Cell Biology

Article Title: Neuregulin 1/ErbB4 signaling attenuates neuronal cell damage under oxygen-glucose deprivation in primary hippocampal neurons

doi: 10.5115/acb.19.210

Figure Lengend Snippet: Neuregulin-1 (NRG1) attenuates primary hippocampal cell death induced by oxygen-glucose deprivation (OGD). NRG1 (5 nM) attenuates cell death induced by OGD in primary hippocampal neurons. After 2 hours, degrees of cell death were assessed by lactate dehydrogenase activity in the medium (n=6, ** P <0.01, # P <0.05). LDH, lactate dehydrogenase.

Article Snippet: The NRG1 used in this study was a recombinant polypeptide containing the entire EGF domain of the β-type NRG1 from PROSPEC (East Brunswick, NJ, USA).

Techniques: Activity Assay